Library Preparation

Convert your sample into sequence libraries

We facilitate the following library preparation methods using standard Illumina reagents. For turn around times, see our FAQ.

Available kits

  • TruSeq DNA Nano LT
  • TruSeq Stranded total RNA, ribo zero for human/mouse/rat
  • TruSeq Stranded mRNA polyA

A maximum of 24 (single index) or 96 (dual index) TruSeq indexes is available. Please contact us if you would like to use custom indexes. Bioinformatics demultiplexing service is supported for a restricted set of indexes.

Sample requirements

All concentrations should be measured with a fluorometric quantitation analysis (e.g. Qubit).

For DNA library preparation:

  • a minimum of 50 μl at a minimal concentration of 4 ng/μl.

For RNA library preparation:

  • RiboZero depletion: a minimum of 12 μl at a minimal concentration of 17 ng/μl.
  • PolyA enrichment: a minimum of 50 μl at a minimal concentration of 4 ng/μl.
  • RNA quality should be at least RIN 8 (RNA Integrity Number)
  • We strongly advise to include DNase treatment during the RNA isolation process.

Requester is responsible for collection of primary sample. Note that samples will be stored for a maximum of 2 months and subsequently discarded without further notice.

Pricing

ServiceTypePrice perPrice (excl VAT)
Truseq DNA nanoSample prepSample€ 83
Truseq RNA stranded polyASample prepSample€ 92
Truseq RNA stranded RiboZero (human, mouse, rat)Sample prepSample€ 150
DNA isolationIsolationSample€ 17
RNA isolationIsolationSample€ 18

Service fees include costs for chemicals, operator time and troubleshooting.


Disclaimer


Library quality is mainly influenced by sample input quality. Users are responsible for ensuring that the quality of primary samples is high enough for successful library preparation. We will contact users when we have any doubt about input or library quality. Reagents that have been used will be charged, even if samples cannot be prepped and/or sequenced.


 

Consultancy Service

For expert advice on your NGS-based research and the possibilities USEQ offers to get the most out of your samples: request a meeting at our consulting hour, each Friday at 1:30pm. Experimental design, technology, platform and library preparation choice, standard bioinformatics analysis, results and follow-up experiments are discussed. The facility manager ensures to bring the right experts from any of the participating organisations to the table.

You can request a meeting at useq@umcutrecht.nl.

NEWS

Nanopore sequencing now available; please contact us for the options!
Due to corona preventive measures we could experience some delays. We will try to minimize it. Apologies for this.
Due to corona preventive measures, sample dropoff access is limited to UMCU staff only. If you are from other institutes, please contact useq and make an appointent so we can personally accept your samples.
NEW: we will add nanostring nCounter to USEQ services in the next few weeks!
PRICE UPDATE: from april 21st we have new prices! Generally 6% lower than 2019.