Convert your sample into sequence libraries
We facilitate the following library preparation methods using standard Illumina reagents. For turn around times, see our FAQ.
- TruSeq DNA Nano LT
- TruSeq Stranded total RNA, ribo zero for human/mouse/rat
- TruSeq Stranded mRNA polyA
A maximum of 24 (single index) or 96 (dual index) TruSeq indexes is available. Please contact us if you would like to use custom indexes. Bioinformatics demultiplexing service is supported for a restricted set of indexes.
All concentrations should be measured with a fluorometric quantitation analysis (e.g. Qubit).
For DNA library preparation:
- a minimum of 50 μl at a minimal concentration of 4 ng/μl.
For RNA library preparation:
- RiboZero depletion: a minimum of 12 μl at a minimal concentration of 17 ng/μl.
- PolyA enrichment: a minimum of 50 μl at a minimal concentration of 4 ng/μl.
- RNA quality should be at least RIN 8 (RNA Integrity Number)
- We strongly advise to include DNase treatment during the RNA isolation process.
Requester is responsible for collection of primary sample. Note that samples will be stored for a maximum of 2 months and subsequently discarded without further notice.
|Service||Type||Price per||Price (excl VAT)|
|Truseq DNA nano||Sample prep||Sample||€ 83|
|Truseq RNA stranded polyA||Sample prep||Sample||€ 92|
|Truseq RNA stranded RiboZero (human, mouse, rat)||Sample prep||Sample||€ 150|
|Nanopore||Sample prep||Sample||€ 60|
|DNA isolation||Isolation||Sample||€ 17|
|RNA isolation||Isolation||Sample||€ 18|
Service fees include costs for chemicals, operator time and troubleshooting.
Library quality is mainly influenced by sample input quality. Users are responsible for ensuring that the quality of primary samples is high enough for successful library preparation. We will contact users when we have any doubt about input or library quality. Reagents that have been used will be charged, even if samples cannot be prepped and/or sequenced.
For expert advice on your NGS-based research and the possibilities USEQ offers to get the most out of your samples: request a meeting at our consulting hour, each Friday at 1:30pm. Experimental design, technology, platform and library preparation choice, standard bioinformatics analysis, results and follow-up experiments are discussed. The facility manager ensures to bring the right experts from any of the participating organisations to the table.
You can request a meeting at firstname.lastname@example.org.