DNA / RNA isolation
from cells, blood, tissue etc
Obtaining high quality sequencing data starts with high quality DNA and RNA. We use the Qiagen QiaSymphony SP for its proven robust high quality automated isolation of whole tissue, cells, organoids, blood. Standard services include human and mouse samples. Please contact us to discuss possibilities for samples of other species. For turn around times, see our FAQ.
Sample requirements
We request samples to be submitted as either snapfrozen tissue, dry cell / organoid pellet, or dissolved in 200 ul ATL buffer (for DNA), dissolved in 400 ul RLT+ buffer (for RNA, stored at -80C).
Requester is responsible for collection of primary sample. Note that samples will be stored for a maximum of 2 months and subsequently discarded without further notice.
Pricing
| Service | Type | Price per | Price (excl VAT) |
|---|---|---|---|
| DNA isolation | Isolation | Sample | € 19 |
| RNA isolation | Isolation | Sample | € 20 |
Service fees include costs for chemicals, operator time and troubleshooting.
Consultancy Service
For expert advice on your NGS-based research and the possibilities USEQ offers to get the most out of your samples: request a meeting at our consulting hour, each Friday at 1:30pm. Experimental design, technology, platform and library preparation choice, standard bioinformatics analysis, results and follow-up experiments are discussed. The facility manager ensures to bring the right experts from any of the participating organisations to the table.
You can request a meeting at useq@umcutrecht.nl.